Blast Sigenae 8 against alpha_v0.3.2


Output
http://aquacul4.fish.washington.edu/~steven/filefish/sigenae8_blastn_v032.out



Blast2GFF

converting blastn to gff
step1
make blast more accurate…

original ~4,700,000 lines


c3>=90


evalue < 1e-10
c11<=1e-10 

algn length >100


Galaxy62-[Filter_on_data_61].tabular





code

./2_Blast2Gff.pl -i /Volumes/Bay3\ scratch/gff_fun/Sig8_blast_v032 -o /Volumes/Bay3\ scratch/gff_fun/cgigas_v032_1.gff -d "sigenae_v8" -p EXON -s "something"


Annotation1_cg_v032.gff